Projetizr is a project mainly written in JavaScript, it's free.
Projetizr is a web tool created to facilitate the process of implementing an HTML layout
This javascript plugin was developed thinking about the workflow behind convering a template to its HTML4/5 counterpart.
The base concept is simple, being able to develop pixel perfect templates.
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Keys: Esc: Reset to default position Alt + Mouse left click: Move background on x axis Alt + Mouse right click: Move background on y axis Alt + Both mouse buttons: Move background on x and y axis Shift + Click: Choose a scope in where to confine your background image r: Activate rulers (might take a few seconds to load) Ctrl+g: Activate guides while in ruler view
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Beta:
This is still in early beta and only works properly on Google Chrome.
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Examples
You can see it in action here: http://www.joseairosa.com/projetizr